Analysis Note

ControlPOSITIVE CONTROL:

embryonic or early post-natal rat brain.

Application

Research Sub CategoryGrowth Cones & Axon Guidance

Western blot. Recognizes bands at 250, 190, and 180 kDa on western blots of embryonic rat brain extracts.

Immunocytochemistry: 1:10

Immunohistochemistry on 4% paraformaldehyde fixed tissue: 1:1,000

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Anti-Phosphacan Antibody, clone 122.2 detects level of Phosphacan & has been published & validated for use in WB, IC, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Phosphacan is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Ascites fluid. Liquid. Contains no preservative.

Specificity

Phosphacan (Receptor Tyrosine Phosphatase Beta). Recognizes the core protein.

Storage and Stability

Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.